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Evolutionary Consistency Analysis

Upload an A3M file (MSA) to analyze evolutionary consistency of generated sequences. The MSA should contain homologous sequences for the target protein family.

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Run ESMFold

Select folding mode and run structure prediction for generated sequences.

Randomly select N sequences to fold
Status:

Create Analysis Workspace

Upload a FASTA file to create an analysis workspace. You can then run structure prediction, evolutionary consistency analysis, and other evaluation tools on your sequences.

Leave blank to use Job ID as name
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Upload sequences to analyze (supports .fasta, .fa, .faa, .fas)

Upload Structure File

Upload a PDB or mmCIF file for inverse folding. Select the chain to use for sequence design.

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Length: -
Sequence: -
Parsing structure file...

Compute SS3 from Structure

Upload a PDB/mmCIF file to compute secondary structure (SS3) via DSSP.

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Length: -
SS3 Preview: -
Computing SS3...

SS3 from AlphaFold DB

Fetch secondary structure from AlphaFold Database using a UniProt ID.

Auto-filled from Reference Protein ID if available.
Length: -
SS3: -
Fetching SS3 from AlphaFold DB...

Delete Job

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Rename Job

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Delete All Jobs

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Compute MSA Profile?

MSA file uploaded successfully. Would you like to compute the MSA profile now?